Year: 2016 | Month: February | Volume 6 | Issue 1
Detection of Anti Mycoplasma gallisepticum Antibodies in Different Age Group of Chicken by Enzyme Linked Immunosorbant Assay
Abstract:
Mycoplasma gallisepticum is an important Mycoplasma species, causes chronic respiratory disease in poultry. Present study was conducted with the aim of detection of anti Mycoplasma gallisepticum antibodies in different age group of chicken by enzyme linked immunosorbent assay. Chicken serum samples were collected from different areas and unorganized farm of district Rewa (Madhya Pradesh). A solid phase blocking ELISA test was performed in serum samples using SVANOVIR MG-Ab, Sweden kit. Around 98 serum samples of different age group of chicken were collected. Chicken were divided into four different age groups viz. group I (6-24 Wks), II (25-42 Wks), III (43-60 Wks) and IV (61-77 Wks), each group further divided into three subgroups according to age. Age wise study of Mycoplasma gallisepticum antibody detection revealed that age group I (6-24 wk age), II (25-42 Wk age), III (43-60 Wks) and IV(61-77 wk) were showing 30%, 20.83%, 20.83% and 12.50% seroconversion respectively. Study revealed that age group 6-24wks showing maximum antibody titer and age group 61-77 wks showing minimum antibody titer. Age subgroup 6-12 wks showing maximum 40% antibody titer. In overall study, Out of 98 samples tested 21.40% samples were positive by ELISA test. ELISA is used as a highly specific test for the detection of anti MG antibodies in chicken serum.
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